Unknown

Dataset Information

0

Cloning and expression of porcine Colony Stimulating Factor-1 (CSF-1) and Colony Stimulating Factor-1 Receptor (CSF-1R) and analysis of the species specificity of stimulation by CSF-1 and Interleukin 34.


ABSTRACT: Macrophage Colony Stimulating Factor (CSF-1) controls the survival, differentiation and proliferation of cells of the mononuclear phagocyte system. A second ligand for the CSF-1R, Interleukin 34 (IL-34), has been described, but its physiological role is not yet known. The domestic pig provides an alternative to traditional rodent models for evaluating potential therapeutic applications of CSF-1R agonists and antagonists. To enable such studies, we cloned and expressed active pig CSF-1. To provide a bioassay, pig CSF-1R was expressed in the factor-dependent Ba/F3 cell line. On this transfected cell line, recombinant porcine CSF-1 and human CSF-1 had identical activity. Mouse CSF-1 does not interact with the human CSF-1 receptor but was active on pig. By contrast, porcine CSF-1 was active on mouse, human, cat and dog cells. IL-34 was previously shown to be species-specific, with mouse and human proteins demonstrating limited cross-species activity. The pig CSF-1R was equally responsive to both mouse and human IL-34. Based upon the published crystal structures of CSF-1/CSF-1R and IL34/CSF-1R complexes, we discuss the molecular basis for the species specificity.

SUBMITTER: Gow DJ 

PROVIDER: S-EPMC3500696 | BioStudies | 2012-01-01

REPOSITORIES: biostudies

Similar Datasets

2013-01-01 | S-EPMC3573236 | BioStudies
1000-01-01 | S-EPMC5552759 | BioStudies
2016-01-01 | S-EPMC5127567 | BioStudies
2012-01-01 | S-EPMC3372767 | BioStudies
1000-01-01 | S-EPMC2924605 | BioStudies
2014-01-01 | S-EPMC3868188 | BioStudies
2012-01-01 | S-EPMC3388946 | BioStudies
2011-01-01 | S-EPMC3212567 | BioStudies
2019-01-01 | S-EPMC6781012 | BioStudies
1000-01-01 | S-EPMC2775986 | BioStudies