Unknown

Dataset Information

0

Structure of the ?-1,6/?-1,4-specific glucansucrase GTFA from Lactobacillus reuteri 121.


ABSTRACT: The reuteransucrase GTFA from Lactobacillus reuteri 121, which belongs to glycosyl hydrolase family GH70, synthesizes branched ?-glucans with both ?-1,6- and ?-1,4-glycosidic linkages (reuteran) from sucrose. The crystal structure of GTFA-?N, a 118?kDa fragment of GTFA comprising residues 745-1763 and including the catalytic domain, was determined at 3.6?Å resolution by molecular replacement. The crystals have large solvent channels and an unusually high solvent content of 85%. GTFA-?N has the same domain arrangement and domain topologies as observed in previously determined GH70 glucansucrase structures. The architecture of the GTFA-?N active site and binding pocket confirms that glucansucrases have a conserved substrate specificity for sucrose. However, this first crystal structure of an ?-1,6/?-1,4-specific glucansucrase shows that residues from conserved sequence motif IV (1128-1136 in GTFA-?N) contribute to the acceptor-binding subsites and that they display differences compared with other structurally characterized glucansucrases. In particular, the structure clarifies the importance of residues following the transition-state stabilizer for product specificity, and especially residue Asn1134, which is in a position to interact with sugar units in acceptor subsite +2.

SUBMITTER: Pijning T 

PROVIDER: S-EPMC3509963 | BioStudies | 2012-01-01

REPOSITORIES: biostudies

Similar Datasets

1000-01-01 | S-EPMC5442262 | BioStudies
2016-01-01 | S-EPMC4711130 | BioStudies
2013-01-01 | S-EPMC3536977 | BioStudies
2016-01-01 | S-EPMC5066211 | BioStudies
2016-01-01 | S-EPMC4980424 | BioStudies
2014-01-01 | S-EPMC4239627 | BioStudies
2017-01-01 | S-EPMC5216370 | BioStudies
2012-01-01 | S-EPMC3318707 | BioStudies
2004-01-01 | S-EPMC532428 | BioStudies
2016-01-01 | S-EPMC4919382 | BioStudies