Dataset Information


Latent TGF-β binding protein 4 promotes elastic fiber assembly by interacting with fibulin-5.

ABSTRACT: Elastic fiber assembly requires deposition of elastin monomers onto microfibrils, the mechanism of which is incompletely understood. Here we show that latent TGF-β binding protein 4 (LTBP-4) potentiates formation of elastic fibers through interacting with fibulin-5, a tropoelastin-binding protein necessary for elastogenesis. Decreased expression of LTBP-4 in human dermal fibroblast cells by siRNA treatment abolished the linear deposition of fibulin-5 and tropoelastin on microfibrils. It is notable that the addition of recombinant LTBP-4 to cell culture medium promoted elastin deposition on microfibrils without changing the expression of elastic fiber components. This elastogenic property of LTBP-4 is independent of bound TGF-β because TGF-β-free recombinant LTBP-4 was as potent an elastogenic inducer as TGF-β-bound recombinant LTBP-4. Without LTBP-4, fibulin-5 and tropoelastin deposition was discontinuous and punctate in vitro and in vivo. These data suggest a unique function for LTBP-4 during elastic fibrogenesis, making it a potential therapeutic target for elastic fiber regeneration.

PROVIDER: S-EPMC3581912 | BioStudies |

REPOSITORIES: biostudies

Similar Datasets

| S-EPMC6789559 | BioStudies
| S-EPMC3015050 | BioStudies
| S-EPMC1933399 | BioStudies
| S-EPMC2782046 | BioStudies
| S-EPMC2880191 | BioStudies
| S-EPMC2064089 | BioStudies
| S-EPMC186672 | BioStudies
| S-EPMC4156930 | BioStudies
2005-01-01 | S-EPMC1186687 | BioStudies
| S-EPMC2719323 | BioStudies