Unknown

Dataset Information

0

Wide field-of-view on-chip Talbot fluorescence microscopy for longitudinal cell culture monitoring from within the incubator.


ABSTRACT: Time-lapse or longitudinal fluorescence microscopy is broadly used in cell biology. However, current available time-lapse fluorescence microscopy systems are bulky and costly. The limited field-of-view (FOV) associated with the microscope objective necessitates mechanical scanning if a larger FOV is required. Here we demonstrate a wide FOV time-lapse fluorescence self-imaging Petri dish system, termed the Talbot Fluorescence ePetri, which addresses these issues. This system's imaging is accomplished through the use of the Fluorescence Talbot Microscopy (FTM). By incorporating a microfluidic perfusion subsystem onto the platform, we can image cell cultures directly from within an incubator. Our prototype has a resolution limit of 1.2 ?m and an FOV of 13 mm(2). As demonstration, we obtained time-lapse images of HeLa cells expressing H2B-eGFP. We also employed the system to analyze the cells' dynamic response to an anticancer drug, camptothecin (CPT). This method can provide a compact and simple solution for automated fluorescence imaging of cell cultures in incubators.

SUBMITTER: Han C 

PROVIDER: S-EPMC3587116 | BioStudies | 2013-01-01

REPOSITORIES: biostudies

Similar Datasets

2013-01-01 | S-EPMC3726246 | BioStudies
2012-01-01 | S-EPMC3261056 | BioStudies
2015-01-01 | S-EPMC4631490 | BioStudies
1000-01-01 | S-EPMC5504007 | BioStudies
2021-01-01 | S-EPMC7815909 | BioStudies
2015-01-01 | S-EPMC4683435 | BioStudies
2014-01-01 | S-EPMC4321697 | BioStudies
1000-01-01 | S-EPMC4763285 | BioStudies
2016-01-01 | S-EPMC4879638 | BioStudies
2014-01-01 | S-EPMC4138516 | BioStudies