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The circadian binding of CLOCK protein to the promoter of C/ebp? gene in mouse cells.


ABSTRACT: C/EBP? plays important roles in metabolism as well as in the maintenance of energy homeostasis. Here we describe loss of the circadian oscillation of C/ebp? expression in liver of Clock mutant mice. Reporter assays indicate Clock and Bmal significantly induced C/ebp? gene expression whereas Cry suppressed. Real time reporter assays showed that two mutated E-boxes disrupted C/ebp? promoter dependent-oscillation. Chromatin immunoprecipitation suggests Clock can bind to two E-boxes in the C/ebp? promoter with a circadian manner in vivo. Thus, C/ebp? gene transcription is under circadian control of a core clock component, Clock. The data suggests that circadian disturbances may affect metabolic abnormalities through the C/ebp? pathway in liver.

SUBMITTER: Kawasaki H 

PROVIDER: S-EPMC3594305 | BioStudies | 2013-01-01

REPOSITORIES: biostudies

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