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PKC? counteracts oxidative stress by regulating Hsc70 in an esophageal cancer cell line.


ABSTRACT: Using a glutathione S-transferase pull-down liquid chromatography-coupled tandem mass spectrometry approach and immunoprecipitation/immunoblot analysis, we found that heat shock cognate protein 70 (Hsc70) was involved in the complex formed by atypical protein kinase C? (PKC?) and LC3 in the esophageal cancer cell line KYSE30. Further study indicated that Hsc70 was targeted by autophagic degradation, and knockdown of PKC? down-regulated Hsc70 by promoting autophagy. PKC? knockdown sensitized cells to oxidative stress-induced apoptosis, whereas forced PKC? expression counteracted the oxidative stress-induced apoptosis via Hsc70.

SUBMITTER: Wang BS 

PROVIDER: S-EPMC3631091 | BioStudies | 2013-01-01T00:00:00Z

REPOSITORIES: biostudies

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