Unknown

Dataset Information

0

Purification of pregnancy-associated glycoproteins from late-pregnancy Bubalus bubalis placentas and development of a radioimmunoassay for pregnancy diagnosis in water buffalo females.


ABSTRACT: BACKGROUND: Pregnancy-associated glycoproteins (PAGs) were first described as placental antigens present in the blood serum of the mother soon after implantation. Here, we describe the purification of several pregnancy-associated glycoproteins from water buffalo placenta (wbPAGs). A specific radioimmunoassay (RIA) was developed for early pregnancy diagnosis in buffalo species. RESULTS: Amino-terminal microsequencing of immunoreactive placental proteins allowed the identification of eleven wbPAGs sequences [Swiss-Prot accession numbers: P86369 to P86379]. Three polyclonal antisera (AS#858, AS#859 and AS#860) were raised in rabbits against distinct wbPAG fractions. A new RIA (RIA-860) was developed and used to distinguish between pregnant (n=33) and non-pregnant (n=26) water buffalo females. CONCLUSIONS: Our results confirmed the multiplicity of PAG expression in buffalo placenta. In addition, the RIA-860 system was shown to be sensitive, linear, reproducible, accurate and specific in measuring PAG concentrations in buffalo plasma samples from Day 37 of gestation onwards.

PROVIDER: S-EPMC3661400 | BioStudies |

REPOSITORIES: biostudies

Similar Datasets

| S-EPMC3335542 | BioStudies
| S-EPMC7552302 | BioStudies
| S-EPMC6895653 | BioStudies
| S-EPMC3896668 | BioStudies
| S-EPMC8636505 | BioStudies
| S-EPMC24220 | BioStudies
| S-EPMC5979379 | BioStudies
| S-EPMC6221303 | BioStudies
| S-EPMC7888492 | BioStudies
| S-EPMC3522032 | BioStudies