Unknown

Dataset Information

0

Characterization of BshA, bacillithiol glycosyltransferase from Staphylococcus aureus and Bacillus subtilis.


ABSTRACT: The first step during bacillithiol (BSH) biosynthesis involves the formation of N-acetylglucosaminylmalate from UDP-N-acetylglucosamine and l-malate and is catalyzed by a GT4 class glycosyltransferase enzyme (BshA). Recombinant Staphylococcus aureus and Bacillus subtilis BshA were highly specific and active with l-malate but the former showed low activity with d-glyceric acid and the latter with d-malate. We show that BshA is inhibited by BSH and similarly that MshA (first enzyme of mycothiol biosynthesis) is inhibited by the final product MSH.

SUBMITTER: Upton H 

PROVIDER: S-EPMC3668096 | BioStudies | 2012-01-01

REPOSITORIES: biostudies

Similar Datasets

2010-01-01 | S-EPMC2943542 | BioStudies
1000-01-01 | S-EPMC2851989 | BioStudies
2014-01-01 | S-EPMC3911838 | BioStudies
2016-01-01 | S-EPMC5954418 | BioStudies
2019-01-01 | S-EPMC6511738 | BioStudies
2011-01-01 | S-EPMC3232311 | BioStudies
2013-01-01 | S-EPMC3960972 | BioStudies
2013-01-01 | S-EPMC4065351 | BioStudies
2009-01-01 | S-EPMC3510479 | BioStudies
2015-01-01 | S-EPMC4705035 | BioStudies