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Three dimensional microfluidics with embedded microball lenses for parallel and high throughput multicolor fluorescence detection.


ABSTRACT: We report a 3D microfluidic device with 32 detection channels and 64 sheath flow channels and embedded microball lens array for high throughput multicolor fluorescence detection. A throughput of 358 400 cells/s has been accomplished. This device is realized by utilizing solid immersion micro ball lens arrays for high sensitivity and parallel fluorescence detection. High refractive index micro ball lenses (n = 2.1) are embedded underneath PDMS channels close to cell detection zones in channels. This design permits patterning high N.A. micro ball lenses in a compact fashion for parallel fluorescence detection on a small footprint device. This device also utilizes 3D microfluidic fabrication to address fluid routing issues in two-dimensional parallel sheath focusing and allows simultaneous pumping of 32 sample channels and 64 sheath flow channels with only two inlets.

SUBMITTER: Fan YJ 

PROVIDER: S-EPMC3765297 | BioStudies | 2013-01-01

SECONDARY ACCESSION(S): 10.1063/1.4818944

REPOSITORIES: biostudies

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