Unknown

Dataset Information

0

Volumetric determination of apolipoprotein stoichiometry of circulating HDL subspecies.


ABSTRACT: Although HDL is inversely correlated with coronary heart disease, elevated HDL-cholesterol is not always protective. Additionally, HDL has biological functions that transcend any antiatherogenic role: shotgun proteomics show that HDL particles contain 84 proteins (latest count), many correlating with antioxidant and anti-inflammatory properties of HDL. ApoA-I has been suggested to serve as a platform for the assembly of these protein components on HDL with specific functions - the HDL proteome. However, the stoichiometry of apoA-I in HDL subspecies is poorly understood. Here we use a combination of immunoaffinity chromatography data and volumetric analysis to evaluate the size and stoichiometry of LpA-I and LpA-I,A-II particles. We conclude that there are three major LpA-I subspecies: two major particles, HDL[4] in the HDL3 size range (d = 85.0 ± 1.2 Å) and HDL[7] in the HDL2 size range (d = 108.5 ± 3.8 Å) with apoA-I stoichiometries of 3 and 4, respectively, and a small minor particle, HDL[1] (d = 73.8 ± 2.1Å) with an apoA-I stoichiometry of 2. Additionally, we conclude that the molar ratio of apolipoprotein to surface lipid is significantly higher in circulating HDL subspecies than in reconstituted spherical HDL particles, presumably reflecting a lack of phospholipid transfer protein in reconstitution protocols.

SUBMITTER: Segrest JP 

PROVIDER: S-EPMC3770086 | BioStudies | 2013-01-01T00:00:00Z

REPOSITORIES: biostudies

Similar Datasets

1000-01-01 | S-EPMC5496035 | BioStudies
2010-01-01 | S-EPMC3006032 | BioStudies
2013-01-01 | S-EPMC3603221 | BioStudies
1000-01-01 | S-EPMC3520517 | BioStudies
2011-01-01 | S-EPMC3079355 | BioStudies
1000-01-01 | S-EPMC3122915 | BioStudies
2016-01-01 | S-EPMC4987197 | BioStudies
2011-01-01 | S-EPMC3096778 | BioStudies
1000-01-01 | S-EPMC2681406 | BioStudies
2020-01-01 | S-EPMC7234989 | BioStudies