Dataset Information


Role of HDL in cholesteryl ester metabolism of lipopolysaccharide-activated P388D1 macrophages.

ABSTRACT: Infections share with atherosclerosis similar lipid alterations, with accumulation of cholesteryl esters (CEs) in activated macrophages and concomitant decrease of cholesterol-HDL (C-HDL). Yet the precise role of HDL during microbial infection has not been fully elucidated. Activation of P388D1 by lipopolysaccharide (LPS) triggered an increase of CEs and neutral lipid contents, along with a remarkable enhancement in 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate-HDL uptake. Similar results were found in human monocyte-derived macrophages and monocytes cocultured with phytohemagglutinin-activated lymphocytes. Inhibition of cholesterol esterification with Sandoz-58035 resulted in 80% suppression of CE biosynthesis in P388D1. However, only a 35% decrease of CE content, together with increased scavenger receptor class B member 1 (SR-B1) protein expression, was found after 72 h and thereafter up to 16 passages of continuous ACAT suppression. Chronic inhibition blunted the effect of LPS treatment on cholesterol metabolism, increased the ratio of free cholesterol/CE content and enhanced interleukin 6 secretion. These results imply that, besides de novo biosynthesis and acquisition by LDL, HDL contributes probably through SR-B1 to the increased CE content in macrophages, partly explaining the low levels of C-HDL during their activation. Our data suggest that in those conditions where more CEs are required, HDL rather than removing, may supply CEs to the cells.


PROVIDER: S-EPMC3793620 | BioStudies | 2013-01-01T00:00:00Z

REPOSITORIES: biostudies

Similar Datasets

2017-01-01 | S-EPMC5448121 | BioStudies
2000-01-01 | S-EPMC1221179 | BioStudies
1000-01-01 | S-EPMC4671069 | BioStudies
1000-01-01 | S-EPMC5129619 | BioStudies
1997-01-01 | S-EPMC1218699 | BioStudies
2011-01-01 | S-EPMC3262050 | BioStudies
2018-01-01 | S-EPMC6233513 | BioStudies
2009-01-01 | S-EPMC2682469 | BioStudies
1000-01-01 | S-EPMC4625174 | BioStudies
1000-01-01 | S-EPMC4203386 | BioStudies