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MicroRNA-143 regulates adipogenesis by modulating the MAP2K5-ERK5 signaling.


ABSTRACT: A better understanding of the molecular mechanisms that regulate adipose tissue-derived stromal cell (ADSC) differentiation could provide new insight into some adipose-tissue-related disease. The differentiation of ADSCs into adipocytes is a complex physiological process that includes clonal expansion, growth arrest, and terminal differentiation. Here the role of microRNA-143 (miR-143) during ADSC adipogenic differentiation was systematically investigated. We found that miR-143 expression was transiently decreased after adipogenic induction while increased from day 3 and peaked on day 7 after induction. We show for the first time that the role of miR-143 is not consistent in the differentiation process. The regulatory role depends on the differentiation stage that miR-143 acts on. When miR-143 is overexpressed during the clonal expansion stage, the adipogenic differentiation of ADSCs is inhibited, whereas the overexpression of miR-143 during the growth arrest stage or terminal differentiation stage promotes differentiation. Further we firstly demonstrate that miR-143 plays the modulational role by directly repressing MAP2K5, a key member of the MAPKK family in the MAPK signaling pathway. These findings suggest that miR-143 plays an important role in adipose tissue formation, with special implications for some metabolic disease in which the amount and/or function of adipose tissue is altered.

SUBMITTER: Chen L 

PROVIDER: S-EPMC3897956 | BioStudies | 2014-01-01

SECONDARY ACCESSION(S): 10.1038/srep03819

REPOSITORIES: biostudies

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MicroRNA-143 regulates adipogenesis by modulating the MAP2K5-ERK5 signaling.

Chen Lin L   Hou Jia J   Ye Lanfeng L   Chen Yuanwei Y   Cui Junhui J   Tian Weidong W   Li Cai C   Liu Lei L  

Scientific reports 20140122


A better understanding of the molecular mechanisms that regulate adipose tissue-derived stromal cell (ADSC) differentiation could provide new insight into some adipose-tissue-related disease. The differentiation of ADSCs into adipocytes is a complex physiological process that includes clonal expansion, growth arrest, and terminal differentiation. Here the role of microRNA-143 (miR-143) during ADSC adipogenic differentiation was systematically investigated. We found that miR-143 expression was tr  ...[more]

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