Dataset Information


Comparative study of efficacy of dopaminergic neuron differentiation between embryonic stem cell and protein-based induced pluripotent stem cell.

ABSTRACT: In patients with Parkinson's disease (PD), stem cells can serve as therapeutic agents to restore or regenerate injured nervous system. Here, we differentiated two types of stem cells; mouse embryonic stem cells (mESCs) and protein-based iPS cells (P-iPSCs) generated by non-viral methods, into midbrain dopaminergic (mDA) neurons, and then compared the efficiency of DA neuron differentiation from these two cell types. In the undifferentiated stage, P-iPSCs expressed pluripotency markers as ES cells did, indicating that protein-based reprogramming was stable and authentic. While both stem cell types were differentiated to the terminally-matured mDA neurons, P-iPSCs showed higher DA neuron-specific markers' expression than ES cells. To investigate the mechanism of the superior induction capacity of DA neurons observed in P-iPSCs compared to ES cells, we analyzed histone modifications by genome-wide ChIP sequencing analysis and their corresponding microarray results between two cell types. We found that Wnt signaling was up-regulated, while SFRP1, a counter-acting molecule of Wnt, was more suppressed in P-iPSCs than in mESCs. In PD rat model, transplantation of neural precursor cells derived from both cell types showed improved function. The present study demonstrates that P-iPSCs could be a suitable cell source to provide patient-specific therapy for PD without ethical problems or rejection issues.

PROVIDER: S-EPMC3899054 | BioStudies |

REPOSITORIES: biostudies

Similar Datasets

| S-EPMC5689771 | BioStudies
| E-GEOD-47178 | BioStudies
| S-EPMC3104759 | BioStudies
| S-EPMC4740755 | BioStudies
2015-01-01 | S-EPMC4620536 | BioStudies
| S-EPMC3578553 | BioStudies
2014-05-13 | E-GEOD-47178 | ArrayExpress
| S-EPMC3775937 | BioStudies
| S-EPMC6789186 | BioStudies
| S-EPMC4860772 | BioStudies