Dataset Information


A palindromic CpG-containing phosphodiester oligodeoxynucleotide as a mucosal adjuvant stimulates plasmacytoid dendritic cell-mediated T(H)1 immunity.



CpG oligodeoxynucleotides (ODNs), resembling bacterial DNA, are currently tested in clinical trials as vaccine adjuvants. They have the nuclease-resistant phosphorothioate bond; the immune responses elicited differ according to the CpG ODN sequence and vaccination method. To develop a CpG ODN that can induce plasmacytoid dendritic cell (pDC)-mediated T(H)1 immunity through the mucosa, we constructed phosphodiester G9.1 comprising one palindromic CpG motif with unique polyguanosine-runs that allows degradation similar to naturally occurring bacterial DNA.


T(H)1 and T(H)2 immunity activation was evaluated by cytokine production pattern and T-bet/GATA-3 ratio in human peripheral blood mononuclear cells and mouse bone marrow cells. Adjuvanticity was evaluated in mice administered G9.1 with diphtheria toxoid (DT) through nasal vaccination.


G9.1 exhibited stronger IFN-?-inducing activity than A-class CpG ODN2216 and increased T-bet/GATA-3 ratio by enhancing T-bet expression. Nasally administered G9.1 plus DT induced DT-specific mucosal IgA and serum IgG, but not IgE, responses with antitoxin activity in C57BL/6 and BALB/c mice, possibly due to IFN/BAFF production. Induction of T(H)1, but not T(H)2-type Abs depended completely on pDCs, the first in vivo demonstration by CpG ODNs.


G9.1 is a promising mucosal adjuvant for induction of pDC-mediated T(H)1 immunity.


PROVIDER: S-EPMC3933336 | BioStudies | 2014-01-01

REPOSITORIES: biostudies

Similar Datasets

2012-01-01 | S-EPMC3356174 | BioStudies
2016-01-01 | S-EPMC4997267 | BioStudies
2012-01-01 | S-EPMC3405294 | BioStudies
1000-01-01 | S-EPMC1976374 | BioStudies
2014-01-01 | S-EPMC4060301 | BioStudies
2012-07-01 | GSE30849 | GEO
1000-01-01 | S-EPMC5074278 | BioStudies
1000-01-01 | S-EPMC6234694 | BioStudies
2013-01-01 | S-EPMC3870712 | BioStudies
2020-01-01 | S-EPMC7015978 | BioStudies