Dataset Information


Single-cell analysis reveals that insulation maintains signaling specificity between two yeast MAPK pathways with common components.

ABSTRACT: Eukaryotic cells use multiple mitogen-activated protein kinase (MAPK) cascades to evoke appropriate responses to external stimuli. In Saccharomyces cerevisiae, the MAPK Fus3 is activated by pheromone-binding heterotrimeric guanosine triphosphate-binding protein (G protein)-coupled receptors to promote mating, whereas the MAPK Hog1 is activated by hyperosmotic stress to elicit the high-osmolarity glycerol (HOG) response. Although these MAPK pathways share several upstream components, exposure to either pheromone or osmolyte alone triggers only the appropriate response. We used fluorescence localization- and transcription-specific reporters to assess activation of these pathways in individual cells on the minute and hour time scale, respectively. Dual activation of these two MAPK pathways occurred over a broad range of stimulant concentrations and temporal regimes in wild-type cells subjected to costimulation. Thus, signaling specificity is achieved through an "insulation" mechanism, not a "cross-inhibition" mechanism. Furthermore, we showed that there was a critical period during which Hog1 activity had to occur for proper insulation of the HOG pathway.

SUBMITTER: Patterson JC 

PROVIDER: S-EPMC3995081 | BioStudies | 2010-01-01

REPOSITORIES: biostudies

Similar Datasets

2013-01-01 | S-EPMC3616637 | BioStudies
2013-01-01 | S-EPMC3701258 | BioStudies
2017-01-01 | S-EPMC5780368 | BioStudies
1999-01-01 | S-EPMC317133 | BioStudies
1000-01-01 | S-EPMC2688530 | BioStudies
2013-01-01 | S-EPMC3540157 | BioStudies
2012-01-01 | S-EPMC3252264 | BioStudies
2006-01-01 | S-EPMC1347046 | BioStudies
2011-01-01 | S-EPMC3104989 | BioStudies
1000-01-01 | S-EPMC2669196 | BioStudies