Unknown

Dataset Information

0

Hydroxyeicosapentaenoic acids from the Pacific krill show high ligand activities for PPARs.


ABSTRACT: PPARs regulate the expression of genes for energy metabolism in a ligand-dependent manner. PPARs can influence fatty acid oxidation, the level of circulating triglycerides, glucose uptake and insulin sensitivity. Here, we demonstrate that 5-hydroxyeicosapentaenoic acid (HEPE), 8-HEPE, 9-HEPE, 12-HEPE and 18-HEPE (hydroxylation products of EPA) obtained from methanol extracts of Pacific krill (Euphausia pacifica) can act as PPAR ligands. Two of these products, 8-HEPE and 9-HEPE, enhanced the transcription levels of GAL4-PPARs to a significantly greater extent than 5-HEPE, 12-HEPE, 18-HEPE, EPA, and EPA ethyl-ester. 8-HEPE also activated significantly higher transcription of GAL4-PPAR?, GAL4-PPAR?, and GAL4-PPAR? than EPA at concentrations greater than 4, 64, and 64 ?M, respectively. We also demonstrated that 8-HEPE increased the expression levels of genes regulated by PPARs in FaO, 3T3-F442A, and C2C12 cells. Furthermore, 8-HEPE enhanced adipogenesis and glucose uptake. By contrast, at the same concentrations, EPA showed weak or little effect, indicating that 8-HEPE was the more potent inducer of physiological effects.

SUBMITTER: Yamada H 

PROVIDER: S-EPMC3995467 | BioStudies | 2014-01-01T00:00:00Z

REPOSITORIES: biostudies

Similar Datasets

2016-01-01 | S-EPMC4864551 | BioStudies
2019-01-01 | S-EPMC6950470 | BioStudies
2011-01-01 | S-EPMC3253701 | BioStudies
1000-01-01 | S-EPMC5481642 | BioStudies
1000-01-01 | S-EPMC4113943 | BioStudies
2017-01-01 | S-EPMC5622761 | BioStudies
2020-01-01 | S-EPMC7497168 | BioStudies
2015-01-01 | S-EPMC4498302 | BioStudies
1000-01-01 | S-EPMC5372002 | BioStudies
2020-01-01 | S-EPMC7461101 | BioStudies