Dataset Information


A regulatory network of Drosophila germline stem cell self-renewal.

ABSTRACT: Stem cells possess the capacity to generate two cells of distinct fate upon division: one cell retaining stem cell identity and the other cell destined to differentiate. These cell fates are established by cell-type-specific genetic networks. To comprehensively identify components of these networks, we performed a large-scale RNAi screen in Drosophila female germline stem cells (GSCs) covering ?25% of the genome. The screen identified 366 genes that affect GSC maintenance, differentiation, or other processes involved in oogenesis. Comparison of GSC regulators with neural stem cell self-renewal factors identifies common and cell-type-specific self-renewal genes. Importantly, we identify the histone methyltransferase Set1 as a GSC-specific self-renewal factor. Loss of Set1 in neural stem cells does not affect cell fate decisions, suggesting a differential requirement of H3K4me3 in different stem cell lineages. Altogether, our study provides a resource that will help to further dissect the networks underlying stem cell self-renewal.

PROVIDER: S-EPMC3998650 | BioStudies |

REPOSITORIES: biostudies

Similar Datasets

| S-EPMC4744108 | BioStudies
| S-EPMC3292427 | BioStudies
| S-EPMC4113433 | BioStudies
| S-EPMC3494938 | BioStudies
2011-01-01 | S-EPMC3245301 | BioStudies
| S-EPMC7058515 | BioStudies
| S-EPMC5987254 | BioStudies
| S-EPMC5666619 | BioStudies
| S-EPMC6499328 | BioStudies
| S-EPMC3841267 | BioStudies