Unknown

Dataset Information

0

Top-down protein identification of proteasome proteins with nanoLC-FT-ICR-MS employing data-independent fragmentation methods.


ABSTRACT: A comparison of different data-independent fragmentation methods combined with LC coupled to high-resolution FT-ICR-MS/MS is presented for top-down MS of protein mixtures. Proteins composing the 20S and 19S proteasome complexes and their PTMs were identified using a 15 T FT-ICR mass spectrometer. The data-independent fragmentation modes with LC timescales allowed for higher duty-cycle measurements that better suit online LC-FT-ICR-MS. Protein top-down dissociation was effected by funnel-skimmer collisionally activated dissociation (FS-CAD) and CASI (continuous accumulation of selected ions)-CAD. The N-termini for 9 of the 14 20S proteasome proteins were found to be modified, and the ?3 protein was found to be phosphorylated; these results are consistent with previous reports. Mass-measurement accuracy with the LC-FT-ICR system for the 20- to 30-kDa 20S proteasome proteins was 1 ppm. The intact mass of the 100-kDa Rpn1 subunit from the 19S proteasome complex regulatory particle was measured with a deviation of 17 ppm. The CASI-CAD technique is a complementary tool for intact-protein fragmentation and is an effective addition to the growing inventory of dissociation methods that are compatible with online protein separation coupled to FT-ICR-MS.

PROVIDER: S-EPMC4045009 | BioStudies |

REPOSITORIES: biostudies

Similar Datasets

| S-EPMC5731941 | BioStudies
| S-EPMC3706383 | BioStudies
| S-EPMC3864674 | BioStudies
| S-EPMC5353847 | BioStudies
| S-EPMC2517000 | BioStudies
| S-EPMC1084299 | BioStudies
| S-EPMC6241286 | BioStudies
| S-EPMC2766596 | BioStudies
| S-EPMC6765334 | BioStudies
| S-EPMC6153970 | BioStudies