Dataset Information


Kinetics of mushroom tyrosinase and melanogenesis inhibition by N-acetyl-pentapeptides.

ABSTRACT: We investigated the kinetics of 4N-acetyl-pentapeptides, Ac-P1, Ac-P2, Ac-P3, and Ac-P4, regarding inhibition of mushroom tyrosinase activity. The peptides sequences of Ac-P1, Ac-P2, Ac-P3, and Ac-P4 were Ac-RSRFK, Ac-KSRFR, Ac-KSSFR, and Ac-RSRFS, respectively. The 4N-acetyl-pentapeptides were able to reduce the oxidation of l-DOPA by tyrosinase in a dose-dependent manner. Of the 4N-acetyl-pentapeptides, only Ac-P4 exhibited lag time (80 s) at a concentration of 0.5 mg/mL. The tyrosinase inhibitory effects of Ac-P4 (IC50 0.29 mg/mL) were more effective than those of Ac-P1, Ac-P2, and Ac-P3, in which IC50s were 0.75 mg/mL, 0.78 mg/mL, and 0.81 mg/mL, respectively. Kinetic analysis demonstrated that all 4N-acetyl-pentapeptides were mixed-type tyrosinase inhibitors. Furthermore, 0.1 mg/mL of Ac-P4 exhibited significant melanogenesis inhibition on B16F10 melanoma cells and was more effective than kojic acid. The melanogenesis inhibition of Ac-P4 was dose-dependent and did not induce any cytotoxicity on B16F10 melanoma cells.


PROVIDER: S-EPMC4130364 | BioStudies | 2014-01-01

REPOSITORIES: biostudies

Similar Datasets

2017-01-01 | S-EPMC5397476 | BioStudies
2019-01-01 | S-EPMC6766919 | BioStudies
1000-01-01 | S-EPMC6274892 | BioStudies
2005-01-01 | S-EPMC1184568 | BioStudies
2013-01-01 | S-EPMC3853751 | BioStudies
2015-01-01 | S-EPMC4721237 | BioStudies
2015-01-01 | S-EPMC4653680 | BioStudies
2018-01-01 | S-EPMC5796246 | BioStudies
2019-01-01 | S-EPMC6812742 | BioStudies
1989-01-01 | S-EPMC1133253 | BioStudies