Dataset Information


Cortical parvalbumin GABAergic deficits with ?7 nicotinic acetylcholine receptor deletion: implications for schizophrenia.

ABSTRACT: Dysfunction of cortical parvalbumin (PV)-containing GABAergic interneurons has been implicated in cognitive deficits of schizophrenia. In humans microdeletion of the CHRNA7 (?7 nicotinic acetylcholine receptor, nAChR) gene is associated with cortical dysfunction in a broad spectrum of neurodevelopmental and neuropsychiatric disorders including schizophrenia while in mice similar deletion causes analogous abnormalities including impaired attention, working-memory and learning. However, the pathophysiological roles of ?7 nAChRs in cortical PV GABAergic development remain largely uncharacterized. In both in vivo and in vitro models, we identify here that deletion of the ?7 nAChR gene in mice impairs cortical PV GABAergic development and recapitulates many of the characteristic neurochemical deficits in PV-positive GABAergic interneurons found in schizophrenia. ?7 nAChR null mice had decreased cortical levels of GABAergic markers including PV, glutamic acid decarboxylase 65/67 (GAD65/67) and the ?1 subunit of GABAA receptors, particularly reductions of PV and GAD67 levels in cortical PV-positive interneurons during late postnatal life and adulthood. Cortical GABAergic synaptic deficits were identified in the prefrontal cortex of ?7 nAChR null mice and ?7 nAChR null cortical cultures. Similar disruptions in development of PV-positive GABAergic interneurons and perisomatic synapses were found in cortical cultures lacking ?7 nAChRs. Moreover, NMDA receptor expression was reduced in GABAergic interneurons, implicating NMDA receptor hypofunction in GABAergic deficits in ?7 nAChR null mice. Our findings thus demonstrate impaired cortical PV GABAergic development and multiple characteristic neurochemical deficits reminiscent of schizophrenia in cortical PV-positive interneurons in ?7 nAChR gene deletion models. This implicates crucial roles of ?7 nAChRs in cortical PV GABAergic development and dysfunction in schizophrenia and other neuropsychiatric disorders.

PROVIDER: S-EPMC4136487 | BioStudies |

REPOSITORIES: biostudies

Similar Datasets

2010-01-01 | S-EPMC2901346 | BioStudies
1000-01-01 | S-EPMC4023157 | BioStudies
2014-01-01 | S-EPMC4034251 | BioStudies
2008-01-01 | S-EPMC2570996 | BioStudies
| S-EPMC4583365 | BioStudies
2017-05-19 | GSE99049 | GEO
2019-01-01 | S-EPMC6882825 | BioStudies
2015-01-01 | S-EPMC4431948 | BioStudies
2016-01-01 | S-EPMC5070049 | BioStudies
2021-01-01 | S-EPMC7804852 | BioStudies