Dataset Information


Elucidation of the preferred routes of C8-vinyl reduction in chlorophyll and bacteriochlorophyll biosynthesis.

ABSTRACT: Most of the chlorophylls and bacteriochlorophylls utilized for light harvesting by phototrophic organisms carry an ethyl group at the C8 position of the molecule, the product of a C8-vinyl reductase acting on a chlorophyll/bacteriochlorophyll biosynthetic precursor. Two unrelated classes of C8-vinyl reductase are known to exist, BciA and BciB, found in the purple phototroph Rhodobacter sphaeroides and the cyanobacterium Synechocystis sp. PCC6803 respectively. We constructed strains of each bacterium with the native C8-vinyl reductase swapped for the other class of the enzyme, and combined these replacements with a series of deletions of the native bch and chl genes. In vivo data indicate that the preferred substrates for both classes of the enzyme is C8-vinyl chlorophyllide, with C8-vinyl protochlorophyllide reduced only under conditions in which this pigment accumulates as a result of perturbed formation of chlorophyllide.


PROVIDER: S-EPMC4214422 | BioStudies | 2014-01-01

REPOSITORIES: biostudies

Similar Datasets

2014-01-01 | S-EPMC4331041 | BioStudies
2013-01-01 | S-EPMC3564038 | BioStudies
2014-01-01 | S-EPMC3931815 | BioStudies
2020-05-26 | PXD003139 | Pride
2009-01-01 | S-EPMC2786518 | BioStudies
1000-01-01 | S-EPMC4432870 | BioStudies
2015-01-01 | S-EPMC4528133 | BioStudies
1000-01-01 | S-EPMC3283191 | BioStudies
1988-01-01 | S-EPMC1148995 | BioStudies
2008-01-01 | S-EPMC2443722 | BioStudies