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Determination of motility forces on isolated chromosomes with laser tweezers.


ABSTRACT: Quantitative determination of the motility forces of chromosomes during cell division is fundamental to understanding a process that is universal among eukaryotic organisms. Using an optical tweezers system, isolated mammalian chromosomes were held in a 1064 nm laser trap. The minimum force required to move a single chromosome was determined to be ? 0.8-5 pN. The maximum transverse trapping efficiency of the isolated chromosomes was calculated as ? 0.01-0.02. These results confirm theoretical force calculations of ? 0.1-12 pN to move a chromosome on the mitotic or meiotic spindle. The verification of these results was carried out by calibration of the optical tweezers when trapping microspheres with a diameter of 4.5-15 µm in media with 1-7 cP viscosity. The results of the chromosome and microsphere trapping experiments agree with optical models developed to simulate trapping of cylindrical and spherical specimens.

SUBMITTER: Khatibzadeh N 

PROVIDER: S-EPMC4215326 | BioStudies | 2014-01-01

REPOSITORIES: biostudies

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