Unknown

Dataset Information

0

Paramecium tetraurelia chromatin assembly factor-1-like protein PtCAF-1 is involved in RNA-mediated control of DNA elimination.


ABSTRACT: Genome-wide DNA remodelling in the ciliate Paramecium is ensured by RNA-mediated trans-nuclear crosstalk between the germline and the somatic genomes during sexual development. The rearrangements include elimination of transposable elements, minisatellites and tens of thousands non-coding elements called internally eliminated sequences (IESs). The trans-nuclear genome comparison process employs a distinct class of germline small RNAs (scnRNAs) that are compared against the parental somatic genome to select the germline-specific subset of scnRNAs that subsequently target DNA elimination in the progeny genome. Only a handful of proteins involved in this process have been identified so far and the mechanism of DNA targeting is unknown. Here we describe chromatin assembly factor-1-like protein (PtCAF-1), which we show is required for the survival of sexual progeny and localizes first in the parental and later in the newly developing macronucleus. Gene silencing shows that PtCAF-1 is required for the elimination of transposable elements and a subset of IESs. PTCAF-1 depletion also impairs the selection of germline-specific scnRNAs during development. We identify specific histone modifications appearing during Paramecium development which are strongly reduced in PTCAF-1 depleted cells. Our results demonstrate the importance of PtCAF-1 for the epigenetic trans-nuclear cross-talk mechanism.

SUBMITTER: Ignarski M 

PROVIDER: S-EPMC4231744 | BioStudies | 2014-01-01

REPOSITORIES: biostudies

Similar Datasets

2014-01-01 | S-EPMC4132734 | BioStudies
2008-01-01 | S-EPMC2418586 | BioStudies
1000-01-01 | S-EPMC4518040 | BioStudies
2018-05-15 | GSE111525 | GEO
2018-01-01 | S-EPMC6143343 | BioStudies
2014-01-01 | S-EPMC4177680 | BioStudies
2015-06-15 | E-GEOD-68455 | ArrayExpress
2019-01-01 | S-EPMC6642352 | BioStudies
2014-01-01 | S-EPMC4232520 | BioStudies
2012-01-01 | S-EPMC3464196 | BioStudies