Dataset Information


Ionizing irradiation induces acute haematopoietic syndrome and gastrointestinal syndrome independently in mice.

ABSTRACT: The role of bone marrow (BM) and BM-derived cells in radiation-induced acute gastrointestinal (GI) syndrome is controversial. Here we use bone marrow transplantation (BMT), total body irradiation (TBI) and abdominal irradiation (ABI) models to demonstrate a very limited, if any, role of BM-derived cells in acute GI injury and recovery. Compared with WT BM recipients, mice receiving BM from radiation-resistant PUMA KO mice show no protection from crypt and villus injury or recovery after 15 or 12?Gy TBI, but have a significant survival benefit at 12?Gy TBI. PUMA KO BM significantly protects donor-derived pan-intestinal haematopoietic (CD45+) and endothelial (CD105+) cells after IR. We further show that PUMA KO BM fails to enhance animal survival or crypt regeneration in radiosensitive p21 KO-recipient mice. These findings clearly separate the effects of radiation on the intestinal epithelium from those on the BM and endothelial cells in dose-dependent acute radiation toxicity.

SUBMITTER: Leibowitz BJ 

PROVIDER: S-EPMC4327858 | BioStudies | 2014-01-01

REPOSITORIES: biostudies

Similar Datasets

2011-01-01 | S-EPMC3096742 | BioStudies
2019-01-01 | S-EPMC6400303 | BioStudies
1000-01-01 | S-EPMC5794877 | BioStudies
2014-01-01 | S-EPMC4100167 | BioStudies
2008-01-01 | S-EPMC2892934 | BioStudies
2015-01-01 | S-EPMC4536042 | BioStudies
2015-01-01 | S-EPMC4727745 | BioStudies
2018-01-01 | S-EPMC6079366 | BioStudies
2010-01-01 | S-EPMC3076086 | BioStudies
2014-01-01 | S-EPMC3991713 | BioStudies