Unknown

Dataset Information

0

Identification of immunodominant proteins of the microalgae Prototheca by proteomic analysis.


ABSTRACT: Prototheca zopfii associated with bovine mastitis and human protothecosis exists as two genotypes, of which genotype 1 is considered as non-infectious and genotype 2 as infectious. The mechanism of infection has not yet been described. The present study was aimed to identify genotype 2-specific immunodominant proteins. Prototheca proteins were separated using two-dimensional gel electrophoresis. Subsequent western blotting with rabbit hyperimmune serum revealed 28 protein spots. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry analysis resulted in the identification of 15 proteins including malate dehydrogenase, elongation factor 1-alpha, heat shock protein 70, and 14-3-3 protein, which were previously described as immunogenic proteins of other eukaryotic pathogens.

SUBMITTER: Irrgang A 

PROVIDER: S-EPMC4337938 | BioStudies | 2015-01-01

REPOSITORIES: biostudies

Similar Datasets

2016-01-01 | S-EPMC5297694 | BioStudies
2015-01-01 | S-EPMC4586511 | BioStudies
2012-01-01 | S-EPMC3457419 | BioStudies
2018-01-01 | S-EPMC6049027 | BioStudies
2018-01-01 | S-EPMC6156311 | BioStudies
2019-01-01 | S-EPMC6416184 | BioStudies
2020-01-01 | S-EPMC6971270 | BioStudies
2001-01-01 | S-EPMC87772 | BioStudies
2018-01-01 | S-EPMC6168571 | BioStudies
2008-01-01 | S-EPMC2446845 | BioStudies