Unknown

Dataset Information

0

ATP-dependent effector-like functions of RIG-I-like receptors.


ABSTRACT: The vertebrate antiviral innate immune system is often considered to consist of two distinct groups of proteins: pattern recognition receptors (PRRs) that detect viral infection and induce the interferon (IFN) signaling, and effectors that directly act against viral replication. Accordingly, previous studies on PRRs, such as RIG-I and MDA5, have primarily focused on their functions in viral double-stranded RNA (dsRNA) detection and consequent antiviral signaling. We report here that both RIG-I and MDA5 efficiently displace viral proteins pre-bound to dsRNA in a manner dependent on their ATP hydrolysis, and that this activity assists a dsRNA-dependent antiviral effector protein, PKR, and allows RIG-I to promote MDA5 signaling. Furthermore, truncated RIG-I/MDA5 lacking the signaling domain, and hence the IFN stimulatory activity, displaces viral proteins and suppresses replication of certain viruses in an ATP-dependent manner. Thus, this study reveals novel "effector-like" functions of RIG-I and MDA5 that challenge the conventional view of PRRs.

PROVIDER: S-EPMC4427555 | BioStudies |

REPOSITORIES: biostudies

Similar Datasets

2007-01-01 | S-EPMC1913852 | BioStudies
| S-EPMC3248507 | BioStudies
| S-EPMC2224571 | BioStudies
| S-EPMC2665237 | BioStudies
| S-EPMC7672446 | BioStudies
| S-EPMC3543043 | BioStudies
| S-EPMC3877571 | BioStudies
| S-EPMC4178823 | BioStudies
| S-EPMC8169967 | BioStudies
| S-EPMC7952553 | BioStudies