Dataset Information


High salt-diet reduces SLC14A1 gene expression in the choroid plexus of Dahl salt sensitive rats.

ABSTRACT: Elevated Na(+) concentration ([Na(+)]) in the cerebrospinal fluid (CSF) contributes to the development of salt-sensitive hypertension. CSF is formed by the choroid plexus (CP) in cerebral ventricles, and [Na(+)] in CSF is controlled by transporters in CP. Here, we examined the effect of high salt diet on the expression of urea transporters (UTs) in the CP of Dahl S vs Dahl R rats using real time PCR. High salt intake (8%, for 2 weeks) did not alter the mRNA levels of UT-A (encoded by SLC14A2 gene) in the CP of either Dahl S or Dahl R rats. In contrast, the mRNA levels of UT-B (encoded by SLC14A1 gene) were significantly reduced in the CP of Dahl S rats on high salt diet as compared with Dahl R rats or Dahl S rats on normal salt diet. Reduced UT-B expression was associated with increased [Na(+)] in the CSF and elevated mean arterial pressure (MAP) in Dahl S rats treated with high salt diet, as measured by radiotelemetry. High salt diet-induced reduction in UT-B protein expression in the CP of Dahl S rats was confirmed by Western blot. Immunohistochemistry using UT-B specific antibodies demonstrated that UT-B protein was expressed on the epithelial cells in the CP. These data indicate that high salt diet induces elevations in CSF [Na(+)] and in MAP, both of which are associated with reduced UT-B expression in the CP of Dahl S rats, as compared with Dahl R rats. The results suggest that altered UT-B expression in the CP may contribute to an imbalance of water and electrolytes in the CSF of Dahl S rats on high salt diet, thereby leading to alterations in MAP.


PROVIDER: S-EPMC4428960 | BioStudies | 2015-01-01

REPOSITORIES: biostudies

Similar Datasets

2014-01-01 | S-EPMC4324599 | BioStudies
1000-01-01 | S-EPMC296327 | BioStudies
1000-01-01 | S-EPMC4866033 | BioStudies
2007-01-01 | S-EPMC2013885 | BioStudies
2019-01-01 | S-EPMC6339564 | BioStudies
2009-01-01 | S-EPMC2669474 | BioStudies
2010-01-01 | S-EPMC2909934 | BioStudies
1000-01-01 | S-EPMC5621918 | BioStudies
2019-01-01 | S-EPMC6818028 | BioStudies
2013-01-01 | S-EPMC3760736 | BioStudies