Dataset Information


Metabolic engineering of mannitol production in Lactococcus lactis: influence of overexpression of mannitol 1-phosphate dehydrogenase in different genetic backgrounds.

ABSTRACT: To obtain a mannitol-producing Lactococcus lactis strain, the mannitol 1-phosphate dehydrogenase gene (mtlD) from Lactobacillus plantarum was overexpressed in a wild-type strain, a lactate dehydrogenase(LDH)-deficient strain, and a strain with reduced phosphofructokinase activity. High-performance liquid chromatography and (13)C nuclear magnetic resonance analysis revealed that small amounts (<1%) of mannitol were formed by growing cells of mtlD-overexpressing LDH-deficient and phosphofructokinase-reduced strains, whereas resting cells of the LDH-deficient transformant converted 25% of glucose into mannitol. Moreover, the formed mannitol was not reutilized upon glucose depletion. Of the metabolic-engineering strategies investigated in this work, mtlD-overexpressing LDH-deficient L. lactis seemed to be the most promising strain for mannitol production.

PROVIDER: S-EPMC444806 | BioStudies |

REPOSITORIES: biostudies

Similar Datasets

| S-EPMC8315166 | BioStudies
| S-EPMC3187077 | BioStudies
1992-01-01 | S-EPMC257323 | BioStudies
| S-EPMC3215508 | BioStudies
| S-EPMC3701532 | BioStudies
| S-EPMC7331161 | BioStudies
| S-EPMC6291793 | BioStudies
2015-03-26 | BIOMD0000000572 | BioModels
1991-01-01 | S-EPMC207999 | BioStudies
| S-EPMC3076288 | BioStudies