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A simple assay for glutathione in whole blood.


ABSTRACT: A method for detecting glutathione selectively in whole blood deposited on filter paper is described. GSH is fractionated from proteins, hemoglobin and other potentially interfering components and determined using a resorufin-acrylate fluorescent probe. The relative standard deviation is lower than 5% (n = 5). Recoveries of GSH from whole blood are between 94% and 108.6%.

SUBMITTER: Hakuna L 

PROVIDER: S-EPMC4449837 | BioStudies | 2015-01-01

REPOSITORIES: biostudies

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