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Simultaneous determination of trimethoprim and sulfamethoxazole in dried plasma and urine spots.


ABSTRACT: BACKGROUND:Trimethoprim-sulfamethoxazole (TMP-SMX) is an antimicrobial drug combination commonly prescribed in children and adults. The study objectives were to validate and apply an HPLC-MS/MS method to quantify TMP-SMX in dried plasma spots (DPS) and dried urine spots (DUS), and perform a comparability analysis with liquid matrices. RESULTS:For TMP the validated range was 100-50,000 ng/ml for DPS and 500-250,000 ng/ml for DUS; for SMX, the validated range was 1000-500,000 ng/ml for both DPS and DUS. Good agreement was noted between DPS/DUS and liquid plasma and urine samples for TMP, while only modest agreement was observed for SMX in both matrices. CONCLUSION:A precise, accurate and reproducible method was developed to quantify TMP-SMX in DPS and DUS samples.

SUBMITTER: Gonzalez D 

PROVIDER: S-EPMC4455038 | BioStudies | 2015-01-01

REPOSITORIES: biostudies

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