We need your help! If you've ever found our data helpful, please take our impact survey (15 min). Your replies will help keep the data flowing to the scientific community. Please Click here for Survey

Unknown

Dataset Information

0

Feeding a High Concentrate Diet Down-Regulates Expression of ACACA, LPL and SCD and Modifies Milk Composition in Lactating Goats.


ABSTRACT: High concentrate diets are fed to early and mid-lactation stages dairy ruminants to meet the energy demands for high milk production in modern milk industry. The present study evaluated the effects of a high concentrate diet on milk fat and milk composition, especially, cis-9, trans-11 CLA content in milk and gene expression of lactating goats. Eight mid-lactating goats with rumen fistula were randomly assigned into a high concentrate diet (HCD) group and low concentrate diet (LCD) group. High concentrate diet feeding significantly increased lipopolysaccharides (LPS) in plasma and decreased milk fat content, vaccenic acid (VA) and cis-9, trans-11 CLA in milk of the lactating goats. The mRNA expression levels of sterol regulatory element binding protein B 1c (SREBP1c), lipoprotein lipase (LPL), fatty acid synthetase (FASN) and acetyl-CoA carboxylase ? (ACACA, ACC?) involving in lipid metabolism were analyzed, and ACACA and LPL all decreased in their expression level in the mammary glands of goats fed a high concentrate diet. DNA methylation rate of stearoyl-CoA desaturase (SCD) was elevated and decreased, and SCD mRNA and protein expression was reduced significantly in the mammary glands of goats fed a high concentrate diet. In conclusion, feeding a high concentrate diet to lactating goats decreases milk fat and reduced expression of SCD in the mammary gland, which finally induced cis-9, trans-11 CLA content in milk.

SUBMITTER: Tao H 

PROVIDER: S-EPMC4472775 | BioStudies | 2015-01-01

REPOSITORIES: biostudies

Similar Datasets

2017-01-01 | S-EPMC5623059 | BioStudies
2020-01-01 | S-EPMC7132897 | BioStudies
2019-01-01 | S-EPMC6418271 | BioStudies
2013-01-01 | S-EPMC3871605 | BioStudies
2014-08-29 | E-GEOD-50448 | ArrayExpress
2014-01-01 | S-EPMC4002246 | BioStudies
2014-01-01 | S-EPMC4109875 | BioStudies
1000-01-01 | S-EPMC3706325 | BioStudies
1000-01-01 | S-EPMC2547860 | BioStudies
2013-01-01 | S-EPMC3666273 | BioStudies