Dataset Information


Adaptor Protein 1A Facilitates Dengue Virus Replication.

ABSTRACT: Rearrangement of membrane structure induced by dengue virus (DENV) is essential for replication, and requires host cellular machinery. Adaptor protein complex (AP)-1 is a host component, which can be recruited to components required for membrane rearrangement. Therefore, dysfunction of AP-1 may affect membrane organization, thereby decreasing replication of virus in infected cells. In the present study, AP-1-dependent traffic inhibitor inhibited DENV protein expression and virion production. We further clarified the role of AP-1A in the life cycle of DENV by RNA interference. AP-1A was not involved in DENV entry into cells. However, it facilitated DENV RNA replication. Viral RNA level was reduced significantly in Huh7 cells transfected with AP-1A small interfering RNA (siRNA) compared with control siRNA. Transfection of naked DENV viral RNA into Huh7 cells transfected with AP-1A siRNA resulted in less viral RNA and virion production than transfection into Huh7 cells transfected with control siRNA. Huh7 cells transfected with AP-1A siRNA showed greater modification of membrane structures and fewer vesicular packets compared with cells transfected with control siRNA. Therefore, AP-1A may partly control DENV-induced rearrangement of membrane structures required for viral replication.

PROVIDER: S-EPMC4474434 | BioStudies |

REPOSITORIES: biostudies

Similar Datasets

| S-EPMC8664216 | BioStudies
| S-EPMC4584296 | BioStudies
| S-EPMC7166320 | BioStudies
| S-EPMC446091 | BioStudies
| S-EPMC5690425 | BioStudies
| S-EPMC4774189 | BioStudies
| S-EPMC2825209 | BioStudies
| S-EPMC2654722 | BioStudies
| S-EPMC7103389 | BioStudies
| S-EPMC3404111 | BioStudies