Unknown

Dataset Information

0

Comprehensive protein profiling of synovial fluid in osteoarthritis following protein equalization.


ABSTRACT:

Objective

The aim of the study was to characterise the protein complement of synovial fluid (SF) in health and osteoarthritis (OA) using liquid chromatography mass spectrometry (LC-MS/MS) following peptide-based depletion of high abundance proteins.

Design

SF was used from nine normal and nine OA Thoroughbred horses. Samples were analysed with LC-MS/MS using a NanoAcquity™ LC coupled to an LTQ Orbitrap Velos. In order to enrich the lower-abundance protein fractions protein equalisation was first undertaken using ProteoMiner™. Progenesis-QI™ LC-MS software was used for label-free quantification. In addition immunohistochemistry, western blotting and mRNA expression analysis was undertaken on selected joint tissues.

Results

The number of protein identifications was increased by 33% in the ProteoMiner™ treated SF compared to undepleted SF. A total of 764 proteins (462 with?2 significant peptides) were identified in SF. A subset of 10 proteins were identified which were differentially expressed in OA SF. S100-A10, a calcium binding protein was upregulated in OA and validated with western blotting and immunohistochemistry. Several new OA specific peptide fragments (neopeptides) were identified.

Conclusion

The protein equalisation method compressed the dynamic range of the synovial proteins identifying the most comprehensive SF proteome to date. A number of proteins were identified for the first time in SF which may be involved in the pathogenesis of OA. We identified a distinct set of proteins and neopeptides that may act as potential biomarkers to distinguish between normal and OA joints.

SUBMITTER: Peffers MJ 

PROVIDER: S-EPMC4528073 | BioStudies | 2015-01-01

REPOSITORIES: biostudies

Similar Datasets

2022-03-02 | PXD001509 | Pride
2020-01-01 | S-EPMC7341532 | BioStudies
2014-01-01 | S-EPMC4108225 | BioStudies
2020-01-01 | S-EPMC7269004 | BioStudies
2019-01-01 | S-EPMC6390343 | BioStudies
2018-01-01 | S-EPMC5783497 | BioStudies
1000-01-01 | S-EPMC6121933 | BioStudies
2013-01-01 | S-EPMC3618606 | BioStudies
| S-EPMC2871408 | BioStudies
2019-01-01 | S-EPMC6773562 | BioStudies