Unknown

Dataset Information

0

Absence of both Sos-1 and Sos-2 in peripheral CD4(+) T cells leads to PI3K pathway activation and defects in migration.


ABSTRACT: Sos-1 and Sos-2 are ubiquitously expressed Ras-guanine exchange factors involved in Erk-MAP kinase pathway activation. Using mice lacking genes encoding Sos-1 and Sos-2, we evaluated the role of these proteins in peripheral T-cell signaling and function. Our results confirmed that TCR-mediated Erk activation in peripheral CD4(+) T cells does not depend on Sos-1 and Sos-2, although IL-2-mediated Erk activation does. Unexpectedly, however, we show an increase in AKT phosphorylation in Sos-1/2dKO CD4(+) T cells upon TCR and IL-2 stimulation. Activation of AKT was likely a consequence of increased recruitment of PI3K to Grb2 upon TCR and/or IL-2 stimulation in Sos-1/2dKO CD4(+) T cells. The increased activity of the PI3K/AKT pathway led to downregulation of the surface receptor CD62L in Sos-1/2dKO T cells and a subsequent impairment in T-cell migration.

SUBMITTER: Guittard G 

PROVIDER: S-EPMC4536155 | BioStudies | 2015-01-01T00:00:00Z

REPOSITORIES: biostudies

Similar Datasets

2012-01-01 | S-EPMC3321150 | BioStudies
1000-01-01 | S-EPMC3700092 | BioStudies
2018-01-01 | S-EPMC5835342 | BioStudies
2013-01-01 | S-EPMC3574821 | BioStudies
1000-01-01 | S-EPMC2713469 | BioStudies
2016-01-01 | S-EPMC4951228 | BioStudies
1000-01-01 | S-EPMC4209804 | BioStudies
2017-01-01 | S-EPMC5658250 | BioStudies
2012-01-01 | S-EPMC3262879 | BioStudies
2003-01-01 | S-EPMC2172734 | BioStudies