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High-yield isolation of extracellular vesicles using aqueous two-phase system.


ABSTRACT: Extracellular vesicles (EVs) such as exosomes and microvesicles released from cells are potential biomarkers for blood-based diagnostic applications. To exploit EVs as diagnostic biomarkers, an effective pre-analytical process is necessary. However, recent studies performed with blood-borne EVs have been hindered by the lack of effective purification strategies. In this study, an efficient EV isolation method was developed by using polyethylene glycol/dextran aqueous two phase system (ATPS). This method provides high EV recovery efficiency (~70%) in a short time (~15 min). Consequently, it can significantly increase the diagnostic applicability of EVs.

SUBMITTER: Shin H 

PROVIDER: S-EPMC4536486 | BioStudies | 2015-01-01

REPOSITORIES: biostudies

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