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Photo-activatable Cre recombinase regulates gene expression in vivo.


ABSTRACT: Techniques allowing precise spatial and temporal control of gene expression in the brain are needed. Herein we describe optogenetic approaches using a photo-activatable Cre recombinase (PA-Cre) to stably modify gene expression in the mouse brain. Blue light illumination for 12 hours via optical fibers activated PA-Cre in the hippocampus, a deep brain structure. Two-photon illumination through a thinned skull window for 100 minutes activated PA-Cre within a sub-millimeter region of cortex. Light activation of PA-Cre may allow permanent gene modification with improved spatiotemporal precision compared to standard methods.

SUBMITTER: Schindler SE 

PROVIDER: S-EPMC4563371 | BioStudies | 2015-01-01

REPOSITORIES: biostudies

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