Unknown

Dataset Information

0

Generation of functional human pancreatic ? cells in vitro.


ABSTRACT: The generation of insulin-producing pancreatic ? cells from stem cells in vitro would provide an unprecedented cell source for drug discovery and cell transplantation therapy in diabetes. However, insulin-producing cells previously generated from human pluripotent stem cells (hPSC) lack many functional characteristics of bona fide ? cells. Here, we report a scalable differentiation protocol that can generate hundreds of millions of glucose-responsive ? cells from hPSC in vitro. These stem-cell-derived ? cells (SC-?) express markers found in mature ? cells, flux Ca(2+) in response to glucose, package insulin into secretory granules, and secrete quantities of insulin comparable to adult ? cells in response to multiple sequential glucose challenges in vitro. Furthermore, these cells secrete human insulin into the serum of mice shortly after transplantation in a glucose-regulated manner, and transplantation of these cells ameliorates hyperglycemia in diabetic mice.

SUBMITTER: Pagliuca FW 

PROVIDER: S-EPMC4617632 | BioStudies | 2014-01-01

REPOSITORIES: biostudies

Similar Datasets

2014-09-25 | E-GEOD-61714 | ArrayExpress
2020-01-01 | S-EPMC7072676 | BioStudies
2019-01-01 | S-EPMC6372986 | BioStudies
2016-01-01 | S-EPMC5185469 | BioStudies
2017-01-01 | S-EPMC5563509 | BioStudies
2004-01-01 | S-EPMC3422216 | BioStudies
1000-01-01 | S-EPMC5059670 | BioStudies
2007-01-01 | S-EPMC1924909 | BioStudies
2020-01-01 | S-EPMC7433758 | BioStudies
1000-01-01 | S-EPMC3636645 | BioStudies