Dataset Information


A DNA Mini-Barcoding System for Authentication of Processed Fish Products.

ABSTRACT: Species substitution is a form of seafood fraud for the purpose of economic gain. DNA barcoding utilizes species-specific DNA sequence information for specimen identification. Previous work has established the usability of short DNA sequences-mini-barcodes-for identification of specimens harboring degraded DNA. This study aims at establishing a DNA mini-barcoding system for all fish species commonly used in processed fish products in North America. Six mini-barcode primer pairs targeting short (127-314?bp) fragments of the cytochrome c oxidase I (CO1) DNA barcode region were developed by examining over 8,000 DNA barcodes from species in the U.S. Food and Drug Administration (FDA) Seafood List. The mini-barcode primer pairs were then tested against 44 processed fish products representing a range of species and product types. Of the 44 products, 41 (93.2%) could be identified at the species or genus level. The greatest mini-barcoding success rate found with an individual primer pair was 88.6% compared to 20.5% success rate achieved by the full-length DNA barcode primers. Overall, this study presents a mini-barcoding system that can be used to identify a wide range of fish species in commercial products and may be utilized in high throughput DNA sequencing for authentication of heavily processed fish products.

PROVIDER: S-EPMC4626862 | BioStudies | 2015-01-01T00:00:00Z

REPOSITORIES: biostudies

Similar Datasets

2019-01-01 | S-EPMC6573807 | BioStudies
2020-01-01 | S-EPMC7773718 | BioStudies
2015-01-01 | S-EPMC4315593 | BioStudies
2019-01-01 | S-EPMC6614167 | BioStudies
1000-01-01 | S-EPMC2396642 | BioStudies
2020-01-01 | S-EPMC7495058 | BioStudies
2017-01-01 | S-EPMC5446357 | BioStudies
| S-EPMC7526243 | BioStudies
2020-01-01 | S-EPMC7308296 | BioStudies
2018-01-01 | S-EPMC6239289 | BioStudies