Dataset Information


Rapid and Efficient Production of Coronary Artery Ligation and Myocardial Infarction in Mice Using Surgical Clips.

ABSTRACT: The coronary artery ligation model in rodents mimics human myocardial infarction (MI). Normally mechanical ventilation and prolonged anesthesia period are needed. Recently, a method has been developed to create MI by popping-out the heart (without ventilation) followed by immediate suture ligation. Mortality is high due to the time-consuming suture ligation process while the heart is exposed. We sought to improve this method and reduce mortality by rapid coronary ligation using a surgical clip instead of a suture.Mice were randomized into 3 groups: clip MI (CMI), suture MI (SMI), or sham (SHAM). In all groups, heart was manually exposed without intubation through a small incision on the chest wall. Unlike the conventional SMI method, mice in the CMI group received a metal clip on left anterior descending artery (LAD), quickly dispensed by an AutoSuture Surgiclip™. The CMI method took only 1/3 of ligation time of the standard SMI method and improved post-MI survival rate. TTC staining and Masson's trichrome staining revealed a similar degree of infarct size in the SMI and CMI groups. Echocardiograph confirmed that both SMI and CMI groups had a similar reduction of ejection fraction and fraction shortening over the time. Histological analysis showed that the numbers of CD68+ macrophages and apoptotic cells (TUNEL-positive) are indistinguishable between the two groups.This new method, taking only less than 3 minutes to complete, represents an efficient myocardial infarction model in rodents.


PROVIDER: S-EPMC4658059 | BioStudies | 2015-01-01

REPOSITORIES: biostudies

Similar Datasets

2018-01-01 | S-EPMC5757248 | BioStudies
2009-01-01 | S-EPMC2650511 | BioStudies
1000-01-01 | S-EPMC6201221 | BioStudies
2010-01-01 | S-EPMC3005817 | BioStudies
1000-01-01 | S-EPMC5612427 | BioStudies
2014-01-01 | S-EPMC4077784 | BioStudies
2014-01-01 | S-EPMC4281179 | BioStudies
2017-01-01 | S-EPMC5526058 | BioStudies
2020-01-01 | S-EPMC7414702 | BioStudies
2017-11-06 | GSE106472 | GEO