Unknown

Dataset Information

0

Ethosuximide Affects Paired-Pulse Facilitation in Somatosensory Cortex of WAG\Rij Rats as a Model of Absence Seizure.


ABSTRACT: The interaction between somatosensory cortex and thalamus via a thalamocortical loop is a theory behind induction of absence epilepsy. Inside peri-oral somatosensory (S1po) and primary somatosensory forelimb (S1fl) regions, excitatory and inhibitory systems are not balanced and GABAergic inhibitory synapses seem to play a fundamental role in short-term plasticity alterations.We investigated the effects of Ethosuximide on presynaptic changes by utilizing paired-pulse stimulation that was recorded from somatosensory cortex in 18 WAG\Rij rats during epileptic activity. A twisted tripolar electrode including two stimulating electrodes and one recording electrode was implanted into the S1po and S1FL according to stereotaxic landmarks. Paired-pulses (200 µs, 100-1000 µA, 0.1 Hz) were applied to somatosensory cortex at 50, 100, 400, 500 ms inter-pulse intervals for 50 min period.The results showed that paired-pulse facilitation was significantly reduced at all intervals in all times, but compared to the control group of epileptic WAG/Rij rats (p<0.05), it was exceptional about the first 10 minutes after the injection. At the intervals of 50 and 100 ms, a remarkable PPD was found in second, third, fourth and fifth 10-min post injection.These experiments indicate that Ethosuximide has effects on presynaptic facilitation in somatosensory cortex inhibitory loops by alteration in GABA levels that leads to a markedly diminished PPF in paired-pulse stimulation.

SUBMITTER: Ghamkhari Nejad G 

PROVIDER: S-EPMC4729345 | BioStudies | 2015-01-01

SECONDARY ACCESSION(S): 10.5681/apb.2013.057

REPOSITORIES: biostudies

Similar Datasets

2009-01-01 | S-EPMC2712639 | BioStudies
2013-01-01 | S-EPMC3703864 | BioStudies
2015-01-01 | S-EPMC4630678 | BioStudies
2019-01-01 | S-EPMC6427924 | BioStudies
2008-01-01 | S-EPMC3143182 | BioStudies
2012-01-01 | S-EPMC3225716 | BioStudies
2005-01-01 | S-EPMC6725576 | BioStudies
2017-01-01 | S-EPMC5524776 | BioStudies
1000-01-01 | S-EPMC4416582 | BioStudies
2007-01-01 | S-EPMC1925142 | BioStudies