Dataset Information


In vivo longitudinal study of rodent skeletal muscle atrophy using ultrasonography.

ABSTRACT: Muscle atrophy is a widespread ill condition occurring in many diseases, which can reduce quality of life and increase morbidity and mortality. We developed a new method using non-invasive ultrasonography to measure soleus and gastrocnemius lateralis muscle atrophy in the hindlimb-unloaded rat, a well-accepted model of muscle disuse. Soleus and gastrocnemius volumes were calculated using the conventional truncated-cone method and a newly-designed sinusoidal method. For Soleus muscle, the ultrasonographic volume determined in vivo with either method was linearly correlated to the volume determined ex-vivo from excised muscles as muscle weight-to-density ratio. For both soleus and gastrocnemius muscles, a strong linear correlation was obtained between the ultrasonographic volume and the muscle fiber cross-sectional area determined ex-vivo on muscle cryosections. Thus ultrasonography allowed the longitudinal in vivo evaluation of muscle atrophy progression during hindlimb unloading. This study validates ultrasonography as a powerful method for the evaluation of rodent muscle atrophy in vivo, which would prove useful in disease models and therapeutic trials.


PROVIDER: S-EPMC4735519 | BioStudies | 2016-01-01

REPOSITORIES: biostudies

Similar Datasets

2017-01-01 | S-EPMC5530106 | BioStudies
2017-01-01 | S-EPMC5691716 | BioStudies
1983-01-01 | S-EPMC1154193 | BioStudies
2019-01-01 | S-EPMC6770236 | BioStudies
1000-01-01 | S-EPMC4019835 | BioStudies
2015-01-01 | S-EPMC4686181 | BioStudies
2020-01-01 | S-EPMC7072003 | BioStudies
2007-10-06 | E-GEOD-1062 | BioStudies
1000-01-01 | S-EPMC3043802 | BioStudies
2017-01-01 | S-EPMC5451534 | BioStudies