Dataset Information


A new non-canonical pathway of G?(q) protein regulating mitochondrial dynamics and bioenergetics.

ABSTRACT: Contrary to previous assumptions, G proteins do not permanently reside on the plasma membrane, but are constantly monitoring the cytoplasmic surfaces of the plasma membrane and endomembranes. Here, we report that the G?q and G?11 proteins locate at the mitochondria and play a role in a complex signaling pathway that regulates mitochondrial dynamics. Our results provide evidence for the presence of the heteromeric G protein (G?q/11??) at the outer mitochondrial membrane and for G?q at the inner membrane. Both localizations are necessary to maintain the proper equilibrium between fusion and fission; which is achieved by altering the activity of mitofusin proteins, Drp1, OPA1 and the membrane potential at both the outer and inner mitochondrial membranes. As a result of the absence of G?q/11, there is a decrease in mitochondrial fusion rates and a decrease in overall respiratory capacity, ATP production and OXPHOS-dependent growth. These findings demonstrate that the presence of G?q proteins at the mitochondria serves as a physiological function: stabilizing elongated mitochondria and regulating energy production in Drp1 and Opa1 dependent mechanisms. This thereby links organelle dynamics and physiology.


PROVIDER: S-EPMC4745894 | BioStudies | 2014-01-01

REPOSITORIES: biostudies

Similar Datasets

2010-01-01 | S-EPMC2942079 | BioStudies
1000-01-01 | S-EPMC3059922 | BioStudies
1000-01-01 | S-EPMC3920928 | BioStudies
2013-01-01 | S-EPMC3856479 | BioStudies
1000-01-01 | S-EPMC5765209 | BioStudies
2012-01-01 | S-EPMC3396839 | BioStudies
1000-01-01 | S-EPMC2719570 | BioStudies
2014-01-01 | S-EPMC4021475 | BioStudies
2017-01-01 | S-EPMC5562410 | BioStudies
1000-01-01 | S-EPMC3740888 | BioStudies