Dataset Information


Tomato 26S Proteasome subunit RPT4a regulates ToLCNDV transcription and activates hypersensitive response in tomato.

ABSTRACT: Involvement of 26S proteasomal subunits in plant pathogen-interactions, and the roles of each subunit in independently modulating the activity of many intra- and inter-cellular regulators controlling physiological and defense responses of a plant were well reported. In this regard, we aimed to functionally characterize a Solanum lycopersicum 26S proteasomal subunit RPT4a (SlRPT4) gene, which was differentially expressed after Tomato leaf curl New Delhi virus (ToLCNDV) infection in tolerant cultivar H-88-78-1. Molecular analysis revealed that SlRPT4 protein has an active ATPase activity. SlRPT4 could specifically bind to the stem-loop structure of intergenic region (IR), present in both DNA-A and DNA-B molecule of the bipartite viral genome. Lack of secondary structure in replication-associated gene fragment prevented formation of DNA-protein complex suggesting that binding of SlRPT4 with DNA is secondary structure specific. Interestingly, binding of SlRPT4 to IR inhibited the function of RNA Pol-II and subsequently reduced the bi-directional transcription of ToLCNDV genome. Virus-induced gene silencing of SlRPT4 gene incited conversion of tolerant attributes of cultivar H-88-78-1 into susceptibility. Furthermore, transient overexpression of SlRPT4 resulted in activation of programmed cell death and antioxidant enzymes system. Overall, present study highlights non-proteolytic function of SlRPT4 and their participation in defense pathway against virus infection in tomato.


PROVIDER: S-EPMC4890432 | BioStudies | 2016-01-01T00:00:00Z

REPOSITORIES: biostudies

Similar Datasets

2010-01-01 | S-EPMC6640424 | BioStudies
2017-01-01 | S-EPMC6638225 | BioStudies
2017-01-01 | S-EPMC5691616 | BioStudies
2011-01-01 | S-EPMC3125359 | BioStudies
2017-01-01 | S-EPMC5747844 | BioStudies
2013-01-01 | S-EPMC3650187 | BioStudies
2012-01-01 | S-EPMC3550806 | BioStudies
2010-01-01 | S-EPMC2972279 | BioStudies
2015-01-01 | S-EPMC6638473 | BioStudies
2019-01-01 | S-EPMC6460037 | BioStudies