Unknown

Dataset Information

0

New pathogen-specific immunoPET/MR tracer for molecular imaging of a systemic bacterial infection.


ABSTRACT: The specific and rapid detection of Enterobacteriaceae, the most frequent cause of gram-negative bacterial infections in humans, remains a major challenge. We developed a non-invasive method to rapidly detect systemic Yersinia enterocolitica infections using immunoPET (antibody-targeted positron emission tomography) with [64Cu]NODAGA-labeled Yersinia-specific polyclonal antibodies targeting the outer membrane protein YadA. In contrast to the tracer [18F]FDG, [64Cu]NODAGA-YadA uptake co-localized in a dose dependent manner with bacterial lesions of Yersinia-infected mice, as detected by magnetic resonance (MR) imaging. This was accompanied by elevated uptake of [64Cu]NODAGA-YadA in infected tissues, in ex vivo biodistribution studies, whereas reduced uptake was observed following blocking with unlabeled anti-YadA antibody. We show, for the first time, a bacteria-specific, antibody-based, in vivo imaging method for the diagnosis of a Gram-negative enterobacterial infection as a proof of concept, which may provide new insights into pathogen-host interactions.

SUBMITTER: Wiehr S 

PROVIDER: S-EPMC4905453 | BioStudies | 2016-01-01

REPOSITORIES: biostudies

Similar Datasets

1000-01-01 | S-EPMC5596432 | BioStudies
2018-01-01 | S-EPMC6115526 | BioStudies
2016-01-01 | S-EPMC5050101 | BioStudies
2017-01-01 | S-EPMC5209641 | BioStudies
2019-01-01 | S-EPMC6911626 | BioStudies
2017-01-01 | S-EPMC5624854 | BioStudies
2018-01-01 | S-EPMC5906006 | BioStudies
2016-01-01 | S-EPMC5041891 | BioStudies
2012-01-01 | S-EPMC3459285 | BioStudies
2017-01-01 | S-EPMC5624852 | BioStudies