Dataset Information


Highly Branched Pentasaccharide-Bearing Amphiphiles for Membrane Protein Studies.

ABSTRACT: Detergents are essential tools for membrane protein manipulation. Micelles formed by detergent molecules have the ability to encapsulate the hydrophobic domains of membrane proteins. The resulting protein-detergent complexes (PDCs) are compatible with the polar environments of aqueous media, making structural and functional analysis feasible. Although a number of novel agents have been developed to overcome the limitations of conventional detergents, most have traditional head groups such as glucoside or maltoside. In this study, we introduce a class of amphiphiles, the PSA/Es with a novel highly branched pentasaccharide hydrophilic group. The PSA/Es conferred markedly increased stability to a diverse range of membrane proteins compared to conventional detergents, indicating a positive role for the new hydrophilic group in maintaining the native protein integrity. In addition, PDCs formed by PSA/Es were smaller and more suitable for electron microscopic analysis than those formed by DDM, indicating that the new agents have significant potential for the structure-function studies of membrane proteins.


PROVIDER: S-EPMC4906958 | BioStudies | 2016-01-01

REPOSITORIES: biostudies

Similar Datasets

2013-01-01 | S-EPMC3612649 | BioStudies
2010-01-01 | S-EPMC3063152 | BioStudies
2017-01-01 | S-EPMC5858085 | BioStudies
2010-01-01 | S-EPMC3090072 | BioStudies
1000-01-01 | S-EPMC6318253 | BioStudies
2019-01-01 | S-EPMC7220817 | BioStudies
2019-01-01 | S-EPMC7233146 | BioStudies
2017-01-01 | S-EPMC5818263 | BioStudies
2016-01-01 | S-EPMC5500206 | BioStudies
2018-01-01 | S-EPMC7238963 | BioStudies