Unknown

Dataset Information

0

Exosomes mediate cell contact-independent ephrin-Eph signaling during axon guidance.


ABSTRACT: The cellular release of membranous vesicles known as extracellular vesicles (EVs) or exosomes represents a novel mode of intercellular communication. Eph receptor tyrosine kinases and their membrane-tethered ephrin ligands have very important roles in such biologically diverse processes as neuronal development, plasticity, and pathological diseases. Until now, it was thought that ephrin-Eph signaling requires direct cell contact. Although the biological functions of ephrin-Eph signaling are well understood, our mechanistic understanding remains modest. Here we report the release of EVs containing Ephs and ephrins by different cell types, a process requiring endosomal sorting complex required for transport (ESCRT) activity and regulated by neuronal activity. Treatment of cells with purified EphB2(+) EVs induces ephrinB1 reverse signaling and causes neuronal axon repulsion. These results indicate a novel mechanism of ephrin-Eph signaling independent of direct cell contact and proteolytic cleavage and suggest the participation of EphB2(+) EVs in neural development and synapse physiology.

PROVIDER: S-EPMC4932373 | BioStudies |

REPOSITORIES: biostudies

Similar Datasets

| S-EPMC8508685 | BioStudies
2014-01-01 | S-EPMC4594565 | BioStudies
| S-EPMC2329588 | BioStudies
| S-EPMC3947671 | BioStudies
| S-EPMC5814325 | BioStudies
| S-EPMC5550979 | BioStudies
| S-EPMC2890748 | BioStudies
| S-EPMC8025214 | BioStudies
2014-01-01 | S-EPMC4594553 | BioStudies
| S-EPMC6962030 | BioStudies