Unknown

Dataset Information

0

Prolonged Treatment of Peanut-Allergic Mice with Bortezomib Significantly Reduces Serum Anti-Peanut IgE but Does Not Affect Allergic Symptoms.


ABSTRACT: Anti-peanut immunoglobulin E (anti-Pn IgE) can persist throughout life, suggesting that this condition could be maintained by long-lived antibody-secreting cells (ASCs). To determine the role of long-lived ASCs, peanut-allergic mice underwent prolonged treatment with the proteasome inhibitor, bortezomib (Bz).Intravenous Bz was given twice weekly for 21 weeks to peanut-allergic mice. During treatment, serum anti-Pn IgE was measured, and the mice were rechallenged at the end of treatment. Cell populations were measured, and Pn-specific IgG, total IgG, and total IgE ASCs were enumerated in the bone marrow (BM) and spleen (SPL).Prolonged treatment with Bz significantly reduced serum anti-Pn IgE and IgG1 but did not affect symptoms following challenge with Pn, even in mice with undetectable serum anti-Pn IgE. Numbers of CD138+ cells were significantly reduced in the BM but were unaffected in the SPL. Unexpectedly, Bz did not affect numbers of Pn-specific IgG, total IgG, or total IgE ASCs in either the BM or SPL.Cells that maintain long-lived serum anti-Pn IgE are sensitive to Bz. However, prolonged depletion of serum Pn-specific IgE does not result in a decrease of symptoms following challenge with Pn.

SUBMITTER: Moutsoglou DM 

PROVIDER: S-EPMC5102775 | BioStudies | 2016-01-01

REPOSITORIES: biostudies

Similar Datasets

2016-01-01 | S-EPMC4815436 | BioStudies
2012-01-01 | S-EPMC3583068 | BioStudies
2011-01-01 | S-EPMC3145724 | BioStudies
2013-01-01 | S-EPMC3529994 | BioStudies
2016-01-01 | S-EPMC4963300 | BioStudies
1000-01-01 | S-EPMC6258909 | BioStudies
2009-01-01 | S-EPMC2725434 | BioStudies
2011-01-01 | S-EPMC3014995 | BioStudies
2015-01-01 | S-EPMC4437844 | BioStudies
2014-01-01 | S-EPMC4234148 | BioStudies