Unknown

Dataset Information

0

Recombinant cyclophilins lack nuclease activity.


ABSTRACT: Several single-domain prokaryotic and eukaryotic cyclophilins have been identified as also being unspecific nucleases with a role in DNA degradation during the lytic processes that accompany bacterial cell death and eukaryotic apoptosis. Evidence is provided here that the supposed nuclease activity of human and bacterial recombinant cyclophilins is due to contamination of the proteins by the host Escherichia coli endonuclease and is not an intrinsic property of these proteins.

SUBMITTER: Manteca A 

PROVIDER: S-EPMC515149 | BioStudies | 2004-01-01

SECONDARY ACCESSION(S): 10.1128/JB.186.18.6325-6326.2004

REPOSITORIES: biostudies

Similar Datasets

2015-01-01 | S-EPMC4537404 | BioStudies
2020-01-01 | S-EPMC7641896 | BioStudies
2018-01-01 | S-EPMC6085458 | BioStudies
1998-01-01 | S-EPMC22731 | BioStudies
2014-01-01 | S-EPMC3913756 | BioStudies
2017-01-01 | S-EPMC5484177 | BioStudies
2014-01-01 | S-EPMC4132807 | BioStudies
2016-01-01 | S-EPMC4898713 | BioStudies
2016-01-01 | S-EPMC4936150 | BioStudies
2009-01-01 | S-EPMC2679460 | BioStudies