Dataset Information


The metabolic impact of extracellular nitrite on aerobic metabolism of Paracoccus denitrificans.

ABSTRACT: Nitrite, in equilibrium with free nitrous acid (FNA), can inhibit both aerobic and anaerobic growth of microbial communities through bactericidal activities that have considerable potential for control of microbial growth in a range of water systems. There has been much focus on the effect of nitrite/FNA on anaerobic metabolism and so, to enhance understanding of the metabolic impact of nitrite/FNA on aerobic metabolism, a study was undertaken with a model denitrifying bacterium Paracoccus denitrificans PD1222. Extracellular nitrite inhibits aerobic growth of P. denitrificans in a pH-dependent manner that is likely to be a result of both nitrite and free nitrous acid (pKa = 3.25) and subsequent reactive nitrogen oxides generated from the intracellular passage of FNA into P. denitrificans. Increased expression of a gene encoding a flavohemoglobin protein (Fhp) (Pden_1689) was observed in response to extracellular nitrite. Construction and analysis of a deletion mutant established Fhp to be involved in endowing nitrite/FNA resistance at high extracellular nitrite concentrations. Global transcriptional analysis confirmed nitrite-dependent expression of fhp and indicated that P. denitrificans expressed a number of stress response systems associated with protein, DNA and lipid repair. It is therefore suggested that nitrite causes a pH-dependent stress response that is due to the production of associated reactive nitrogen species, such as nitric oxide from the internalisation of FNA.


PROVIDER: S-EPMC5339346 | BioStudies | 2017-01-01

REPOSITORIES: biostudies

Similar Datasets

2018-01-01 | S-EPMC5987163 | BioStudies
2014-01-01 | S-EPMC3907716 | BioStudies
1999-01-01 | S-EPMC94164 | BioStudies
2017-06-30 | GSE97959 | GEO
1000-01-01 | S-EPMC4342570 | BioStudies
2016-01-01 | S-EPMC5007762 | BioStudies
1996-01-01 | S-EPMC1217862 | BioStudies
2001-01-01 | S-EPMC1221906 | BioStudies
2013-11-18 | E-GEOD-48577 | ArrayExpress
| GSE48577 | GEO