Dataset Information


The CEA-/lo colorectal cancer cell population harbors cancer stem cells and metastatic cells.

ABSTRACT: Serum carcinoembryonic antigen (CEA) is the most commonly used tumor marker in a variety of cancers including colorectal cancer (CRC) for tumor diagnosis and monitoring. Recent studies have shown that colonic crypt cells expressing little or no CEA may enrich for stem cells. Numerous studies have clearly shown that there exist CRC patients with normal serum CEA levels during tumor progression or even tumor relapse, although CEA itself is considered to promote metastasis and block cell differentiation. These seemingly contradictory observations prompted us to investigate, herein, the biological properties as well as tumorigenic and metastatic capacity of CRC cells that express high (CEA+) versus low CEA (CEA-/lo) levels of CEA. Our findings show that the abundance of CEA-/lo cells correlate with poor differentiation and poor prognosis, and moreover, CEA-/lo cells form more spheres in vitro, generate more tumors and exhibit a higher potential in developing liver and lung metastases than corresponding CEA+ cells. Applying RNAi-mediated approach, we found that IGF1R mediated tumorigenic and capacity of CEA-/lo cells but did not mediate those of CEA+ cells. Notably, our data demonstrated that CEA molecule was capable of protecting CEA-/lo cells from anoikis, implying that CEA+ cells, although themselves possessing less tumorigenic and metastatic capacity, may promote metastasis of CEA-/lo cells via secreting CEA molecule. Our observations suggest that, besides targeting CEA molecule, CEA-/lo cells may represent a critical source of tumor progression and metastasis, and should therefore be the target of future therapies.


PROVIDER: S-EPMC5348349 | BioStudies | 2016-01-01


REPOSITORIES: biostudies

Similar Datasets

2012-01-01 | S-EPMC3348510 | BioStudies
1000-01-01 | S-EPMC5037276 | BioStudies
2019-01-01 | S-EPMC6828701 | BioStudies
2010-01-01 | S-EPMC2831103 | BioStudies
2020-01-01 | S-EPMC7286116 | BioStudies
2020-01-01 | S-EPMC6985237 | BioStudies
2018-01-01 | S-EPMC6128780 | BioStudies
2019-01-01 | S-EPMC6637112 | BioStudies
2017-01-01 | S-EPMC5732810 | BioStudies
1000-01-01 | S-EPMC5363593 | BioStudies